The fluorescence quantification of polymerase chain reaction (PCR) has revolutionized the field of molecular biology. Through the use of fluorescent dyes and probes, the real-time detection of DNA amplification has become possible, enabling precise quantification of target DNA sequences. This technology has wide-ranging applications in research, clinical diagnostics, and forensic analysis. For example, the fluorescence quantification of PCR has been used to monitor gene expression levels, detect infectious diseases, and identify genetic mutations. The ability to measure DNA amplification in real time with high sensitivity and accuracy has made fluorescence-based PCR a crucial tool in modern biotechnology. This has led to the development of various commercial kits and instruments for quantitative PCR analysis, further expanding the utility of this powerful technique.
荧光定量聚合酶链式反应,Fluorescence quantify polymerase chain reaction
1)Fluorescence quantify polymerase chain reaction荧光定量聚合酶链式反应
1.Objective : To evaluate methods of testing hepatitis B virus deoxyribonucleic acid with Common Polymerase Chain Reaction and Fluorescence Quantify Polymerase Chain Reaction through clinical trial.目的 :评价普通聚合酶链式反应 (C PCR)和荧光定量聚合酶链式反应 (FQ PCR)检测乙型肝炎病毒核酸 (HBVDNA)的临床应用价值。
2)FQ-PCR荧光定量聚合酶链式反应
1.Methods Serum HBV-DNA (withFQ-PCR), HBV markers and Pre-S1 antigen (with ELISA) and ALT, AST (with biochemistry) were determied in 169 patients with hepatitis B.方法:采用荧光定量聚合酶链式反应(FQ-PCR)和ELISA分别检测169例乙肝病人血清HBV-DNA含量和乙肝标志物及Pre-S1Ag与肝功能,并对结果进行对比分析。
3)FQ-PCR荧光定量聚合酶链反应
1.EXPERIMENTAL STUDY ON DETECTION OF HSV-Ⅱ IN PATIENTS WITH STD FROM OUTPATIENT DEPARTMENT WITHFQ-PCR;荧光定量聚合酶链反应检测性病患者感染单纯疱疹病毒Ⅱ的实验研究
2.EXPERIMENTAL STUDY ON DETECTION OF HUMAN PAPILLOMA VIRUSE WITH FLUORESCENT QUANTIVATIVE PCR(FQ-PCR).;荧光定量聚合酶链反应检测人乳头瘤病毒的实验研究
3.Objective To investigate the fluorescence quantitative polymerase chain reaction(FQ-PCR)in the giant cell viral hepatitis in value.目的探讨荧光定量聚合酶链反应(FQ-PCR)在巨细胞病毒性肝炎中的应用价值。
英文短句/例句
1.The accidence exploration of estimation of curative effect in hepatitis B by fluorescence quantitative PCR荧光定量聚合酶链反应对乙型肝炎患者个体用药疗效的评定
2.The Preparation of Satb2 Gene Standard Plasmids for Real-time Quantitative PCR and Its Application;Satb2基因实时荧光定量聚合酶链反应标准品的制备及应用研究
3.Research on the Application in Diagnosis of Fluorescent Quantitation Polymerase Chain Reaction of Paratyphoid A荧光定量聚合酶链反应诊断甲型副伤寒的应用研究
4.Fluorogenic quantitative PCR method for Plasmodium vivax间日疟原虫荧光定量聚合酶链反应检测方法的建立
5.Monitoring of Minimal Residual Disease (MRD) by Real-time Quantitative Reverse Transcription PCR in Acute Promyelocytic Leukemia;实时荧光定量聚合酶链反应对人急性早幼粒细胞白血病的MRD监测
6.Detection of mutans streptococci in children saliva by real-time fluorescence quantitative polymerase chain reaction methods;实时荧光定量聚合酶链反应检测儿童口腔致龋菌的实验研究
7.The assay method used included the Roche Amplicor assay (version 2.0) and real-time PCR.检测方法采用实时荧光定量聚合酶链反应方法和罗氏公司的pcr内标定量方法。
8.Detection of the trachoma chlamydia by fluoroqenic quantitative PCR and super microscope荧光定量聚合酶链反应和超高倍显微诊断系统镜检检测沙眼衣原体
9.Monitoring of P16 Abnormal Methylation by Real-time Fluorescent Quantitative PCR in NSCLC;实时荧光定量聚合酶链反应检测人肺癌P16抑癌基因启动子异常甲基化
10.Clinical evaluation of FQ-PCR assay for early diagnosis of EBV infection in children by testing EBV-DNA in throat washing荧光定量聚合酶链反应法检测咽拭子EBV-DNA在早期诊断EBV感染中的作用
11.Diagnostic Value of Fluorescent Quantitative Polymerase Chain Reaction for Mycoplasma Pneumoniae Pneumoniae in Children with Mycoplasma Pneumoniae Pneumonia肺炎支原体荧光定量聚合酶链反应检测在儿童肺炎支原体肺炎中的诊断意义
12.Detection of mRNA in kidney of dead rat using fluorescence semi-quantitative RT-PCR;荧光半定量逆转录-聚合酶链式反应检测大鼠死后肾mRNA
13.Apply of Real-time Fluorescence Quantitative Polymerase Chain Reaction for the Diagnosis of down Syndrome from a Single Cell;单细胞水平实时荧光定量聚和酶链反应在诊断唐氏综合征中的应用探讨
14.Study of TaqMan assay of real-time fluorescence quantitative PCR to detect Staphylococcus aureus in food实时荧光定量-聚合酶链反应检测食品中金黄色葡萄球菌方法的研究
15.Detection of Campylobacter jejuni by real-time fluorescent PCR实时荧光聚合酶链反应检测空肠弯曲菌
16.Rapid detection of Shigella by real-time fluorescent PCR assay using TaqMan-MGB probes志贺菌TaqMan-MGB探针实时荧光聚合酶链反应快速检测方法的应用研究
17.Establishment of LUX~(TM) real-time fluorescent PCR/RT-PCR for rapid detection of bovine leukemia virus牛白血病病毒LUX~(TM)实时荧光聚合酶链式反应检测方法的建立
18.Development of a SYBR Green Real-time RT-PCR for Detection of Mumps Virus GeneSYBR Green荧光逆转录-聚合酶链反应检测流行性腮腺炎病毒基因
相关短句/例句
FQ-PCR荧光定量聚合酶链式反应
1.Methods Serum HBV-DNA (withFQ-PCR), HBV markers and Pre-S1 antigen (with ELISA) and ALT, AST (with biochemistry) were determied in 169 patients with hepatitis B.方法:采用荧光定量聚合酶链式反应(FQ-PCR)和ELISA分别检测169例乙肝病人血清HBV-DNA含量和乙肝标志物及Pre-S1Ag与肝功能,并对结果进行对比分析。
3)FQ-PCR荧光定量聚合酶链反应
1.EXPERIMENTAL STUDY ON DETECTION OF HSV-Ⅱ IN PATIENTS WITH STD FROM OUTPATIENT DEPARTMENT WITHFQ-PCR;荧光定量聚合酶链反应检测性病患者感染单纯疱疹病毒Ⅱ的实验研究
2.EXPERIMENTAL STUDY ON DETECTION OF HUMAN PAPILLOMA VIRUSE WITH FLUORESCENT QUANTIVATIVE PCR(FQ-PCR).;荧光定量聚合酶链反应检测人乳头瘤病毒的实验研究
3.Objective To investigate the fluorescence quantitative polymerase chain reaction(FQ-PCR)in the giant cell viral hepatitis in value.目的探讨荧光定量聚合酶链反应(FQ-PCR)在巨细胞病毒性肝炎中的应用价值。
4)Fluorescence quantitative polymerase chain reaction荧光定量聚合酶链反应
1.Detection of mutans streptococci in children saliva by real-time fluorescence quantitative polymerase chain reaction methods;实时荧光定量聚合酶链反应检测儿童口腔致龋菌的实验研究
2.Methods 130 patients urogenital tract secretions and prostatic fluid were collected for Uu detection by both broth method and fluorescence quantitative polymerase chain reaction (FQ-PCR), and bacterial culture was conducted in the Uu positive and polluted liquid medium.方法采集130例患者的泌尿生殖道分泌物﹑前列腺液,同时进行液体培养法和荧光定量聚合酶链反应(FQ-PCR)检测Uu,并对Uu阳性和判为污染的液体培养基进行细菌培养。
3.To investigate the HBV DNA,Fluorescence quantitative polymerase chain reaction (FQ-PCR) was performed on 626 samples.方法用酶联免疫吸附试验(ELISA)对51746例临床标本常规HBV检测,对常见模式组用荧光定量聚合酶链反应(FQ—PCR)检测HBVDNA,对罕见模式组用ELISA进行复检。
5)Fluorescence quantitative PCR荧光定量聚合酶链反应
1.Methods:NG -DNA in 121 cases of specimens,CT - DNA and UU - DNA in 149 cases of specimens were examined by Fluorescence Quantitative PCR( FQ - PCR).方法:用荧光定量聚合酶链反应(FQ—PCR)检测了121份标本的NG—DNA和149份标本的CT—DNA与UU—DNA。
6)Fluorescent quantitative polymerase chain reaction荧光定量聚合酶链反应
1.Methods The secretion specimen coming from the high risk patients with CA were examined with fluorescent quantitative polymerase chain reaction (FQ-PCR) for genotype HPV-DNA.方法应用荧光定量聚合酶链反应(FQ-PCR)对尖锐湿疣高危人群分泌物标本进行HPV-DNA分型检测。
2.Objective To explore the significance of fluorescent quantitative polymerase chain reaction(FQ- PCR) assay detecting urine cytomegalovirus(CMV) - DNA to diagnose the cytomegalovirus infection in children.方法应用荧光定量聚合酶链反应 (FQ-PCR)方法检测110例确诊或疑诊CMV感染和81例对照患儿尿CMV-DNA,并与血清抗体检测、pp65抗原检测比较,对更昔洛韦治疗的17例病人进行治疗后尿CMV-DNA;拷贝监测。
3.Methods Expression of NF-κB mRNA andVEGF mRNA in 32 cases gastric cancer and their adjacent noncancerous tissues was measured by fluorescent quantitative polymerase chain reaction(FQ-PCR).方法利用荧光定量聚合酶链反应法测定32例人胃癌细胞和25例癌旁细胞内NF-κBmRNA和VEGFmRNA的定量表达,分析其与临床病理特征的相关性。
延伸阅读
聚合酶链式反应分子式：CAS号：性质：又称基因体外扩增技术或核酸体外扩增技术。利用两种与相反链杂交并利用附着于目标DNA两端的寡核苷酸引物在高温(95℃)使含目标DNA片段的DNA双链变性，分解为单链。在较低温度(37～63℃)与引物退火：然后变温到72℃左右，在耐高温DNA聚合物酶作用下引物被沿样板DNA单链延伸合成互补链，然后又变性→退火→延伸反复进行。引物大大过量，dNTP过量，酶在高温中是较稳定的，这样经过几十个循环，目标DNA片段就按2n数递增。用此法可从mRNA中，cDNA库中扩增出目标基因。过去一般合成500～1000bp较好，现已发展出大片段(75kb)的PCR，且差错甚少。PCR技术的发展还可用于定点突变，质粒重组及FLP等方面。这一技术的作用范围日益扩大，已成为分子生物学工作者基本技术之一。这项专利技术1985年由美国塞特斯(Cetus)公司开发。是20世纪80年代分子生物学领域中的一项革命性突破，已在分子生物学、医学、法学等领域发挥了极大的作用。
