Whole-cell patch clamp is a versatile electrophysiological technique used to study the electrical properties of individual cells. It involves placing a glass pipette filled with an electrolyte solution on the cell membrane to record the ionic currents passing through ion channels. This technique can provide valuable insights into the function of neurons, cardiomyocytes, and other excitable cells. In contrast, the cell-attached and inside-out configurations of the patch clamp technique are used to study the properties of ion channels at the single-channel level. The whole-cell patch clamp technique has revolutionized our understanding of cellular physiology and has contributed significantly to the development of new pharmacological treatments. Its application spans diverse fields, from neuroscience and cardiology to drug discovery and toxicology. Its precision and versatility make it an indispensable tool for researchers in the life sciences.
全细胞膜片钳,whole-cell patch clamp
1)whole-cell patch clamp全细胞膜片钳
1.Methods: Whole-cell patch clamp recording was performed with injured DRG neurons freshly isolated from rats.方法:在急性离散的大鼠DRG中型神经元上,运用全细胞膜片钳技术,观察不同温度梯度刺激下受损DRG神经元的放电的特征。
2.Methods Usingwhole-cell patch clamp techniques to compare the electrophysiological characteristics of ICa.方法经酶解分离获得犬左、右心室外膜下细胞、M细胞和内膜下细胞,应用全细胞膜片钳技术,记录并比较不同部位心肌细胞的ICa。
3.Methods ASICs currents evoked by extracellular solutions of various pH values were recorded and analyzed in acute isolated rat DRG neurons by usingwhole-cell patch clamp technique.方法应用全细胞膜片钳技术,在急性分散的成年大鼠DRG细胞上记录并分析由不同浓度H+(降低pH值)诱发的ASICs电流。
英文短句/例句
1.Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.方法 :采用全细胞膜片钳技术 ,记录钠通道电流。
2.Experiment simulation system based on virtual cells patch clamp基于虚拟细胞的膜片钳实验仿真系统
3.Application of patch clamp whole-cell recording method to study on molecular mechanism of glutamine transporters膜片钳全细胞记录法在研究谷氨酰胺转运蛋白分子机制中的应用
4.Application of General Pippette Solution in Patch-clamp Study on Rabbit Cardiomyocytes通用电极内液在心肌细胞膜片钳实验中的应用
5.Patch-clamp technique was used to observe the changes of sodium channels caused by high concentration glucose.利用膜片钳技术观察高糖条件下Müller细胞Na+通道的变化。
6.Abstract: The study of lens cells by patch clamp technique began in th e early 90"s.文摘:应用膜片钳技术对晶体细胞的研究始于90年代初。
7.Acute Isolation, Culture and the Patch Clamp Study of the Cultured Neurons from Ornithoctonus Huwena;虎纹捕鸟蛛神经细胞急性分离培养及其膜片钳研究
8.The Advances in Researching on Cell Segregation and Culture Technique Suitable for Patch Clamp Technique;适用于膜片钳技术的细胞分离及培养技术的研究进展
9.Isolation and Culture of Nerve Cells from Ornithoctonus huwena and the Patch-clamp Study on the Voltage-gated Ion Channels in the Cultured Neurons虎纹捕鸟蛛神经细胞急性分离培养及其电压门控通道膜片钳
10.Cultural method of dorsal root ganglion neurons of adult rat for patch clamp study一种适合膜片钳记录的成年大鼠背根神经节细胞培养方法
11.Transmembrane ionic channel currents were recorded by using the whole-cell patchclamp technique in single cardiac entricular myocyte enzymatically isolated from guinea-pig hearts.应用全细胞斑片钳技术记录酶学方法分离的豚鼠单个心室肌细胞离子通道电流。
12.Circuit Design of Experimental Control and Data Acquisition for Automatic Patch-Clamp System;全自动膜片钳实验控制和数据采集电路的设计
13.Design and Implementation of Automatic Calibration and Test in Automatic Patch-Clamp Amplifier;全自动膜片钳系统校准和测试的设计与实现
14.Improvement of Transient Artifact Cancellation in Automatic Patch-Clamp Amplifier;全自动膜片钳放大器瞬态伪差信号补偿的改进
15.Analysis and Design of Automatic Patch-Clamp Software System Based on UML;基于UML的全自动膜片钳软件分析与设计
16.USB2.0-based Data Acquisition Designed for Full-automatic Patch-Clamp System;基于USB2.0技术的全自动膜片钳数据采集系统的研制
17.Development of USB Bus Based Data Acquisition and Control Interface for Full-auto Patch-Clamp Systems;基于USB总线技术的全自动膜片钳系统数据采集控制接口的研制
18.The protective effects of transpupillary thermotherapy on retinal ganglion cells in rat with optic nerve crush经瞳孔温热疗法对大鼠视神经钳夹视网膜神经节细胞的保护作用
相关短句/例句
Whole cell patch clamp全细胞膜片钳
1.Methods:Whole cell patch clamp recording was performed on freshly isolated normal DRG neurons and chronically compressed DRG (CCD) neurons before and after the application of NE.方法急性离散大鼠背根节(dorsalrootganglion,DRG)神经元后,以全细胞膜片钳技术记录了正常DRG神经元及慢性压迫DRG神经元的膜电位及放电数目在施加去甲肾上腺素(NE)前后的改变。
2.Method: the whole cell patch clamp recording technique was used in this study.方法 :采用全细胞膜片钳记录法 ,在新鲜分离的大鼠背根神经节细胞上观察血竭对钠通道电流幅值的影响 。
3)whole-cell patch-clamp全细胞膜片钳
1.The effects of scorpion venom heat resistant protein (SVHRP) on sodium channel were studied in freshly isolated hippocampal neurons in rat using thewhole-cell patch-clamp technique.应用全细胞膜片钳技术观察蝎毒耐热蛋白(scorpion venom heat resistant protein,SVHRP)对急性分离大鼠海马神经元电压依赖性钠通道的影响。
2.To explore the characteristics and significances of ATP-activated currents in rat different primary sensory neurons, the experiments were performed on neurons freshly isolated from rat dorsal root ganglion(DRG), trigeminal ganglion (TG) and nodose ganglion (NG) usingwhole-cell patch-clamp.为探讨大鼠不同初级感觉神经元ATP-激活电流的电生理学特征及意义,本实验在新鲜分离的大鼠三种初级感觉神经元标本上,应用全细胞膜片钳技术记录,对外加ATP引起的膜电流的变化进行了研究。
3.Methods Whole-cell patch-clamp technique was used to record the GABA_A receptor-induced currents (I_GABA) in four groups(n=5 of each), ie, propofol(groupⅠ), propofol combined with fentanyl(groupⅡ), midazolam (groupⅢ) and midazolam combined with fentanyl(groupⅣ).方法采用酶-机械法急性分离出生3~10日龄SD大鼠的海马锥体神经元,采用全细胞膜片钳技术记录锥体神经元GABAA受体介导的Cl-电流(GABA电流,IGABA)。
4)Whole-cell Patch-clamp Techniques全细胞膜片钳术
5)Whole cell clamp全细胞式膜片钳
6)whole-cell patch clamp全细胞膜片钳技术
1.In order to investigate the toxic mechanisms of SO2,an air pollutant,in neuronal and cardiovascular systerms,the effects of SO2 derivatives(bisulfite and sulfite,1∶3) on ionic channels in acutely isolated rat hippocampal neurons,dorsal root ganglion(DRG) neurons and cardiomyocytes were studied using thewhole-cell patch clamp technique.为了阐明大气污染物SO2对神经系统和心血管系统的毒作用机制,采用全细胞膜片钳技术研究了SO2衍生物(NaHSO3和Na2SO3,分子比为1∶3)对大鼠海马、背根节神经元和心肌细胞膜上钠、钾、钙离子通道的影响。
2.Methods Whole-cell patch clamp recording technique was used to record I_ Ca,L in single cells isolated rat ventricular myocytes.方法用急性酶解法获得大鼠单个心室肌细胞,用标准全细胞膜片钳技术记录ICa,L,观察不同浓度卡维地洛对ICa,L的影响。
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钳钳1.妄语貌。
